Author(s): S GANTAIT, N. MANDAL, S. BHATTACHARYYA AND P. K. DAS

Abstract: A novel protocol was developed for Allium ampeloprasum L. to enhance in vitro cloning through multiple shoot induction. First bud induction was recorded from shoot tip within 6 days in MS with 0.25 mgl-1 NAA and 2 mgl-1 Kn. A maximum of 3 buds from a single explant appeared within the 15 days after the first bud induction. MS with 2.5 mgl-1 Kn plus 60 mgl-1 of adenine sulphate proved best for multiple shoot proliferation resulting in 6 shoots per inoculated shoot bud within next 30 days. Maximum 5 roots per shoot were recorded when cultured on MS with 0.5 mgl-1 IAA for 20 days. The combination of soil, sand and vermicompost with intermittent water spraying proved to be the best for hardening of micropropagated plantlets ensuring 90% success in next 25 days. Selected ISSR primers were used to ensure genetic clonality for the in vitro generated propagules.

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