Vol. 13 No. 3
An efficient micropropagation protocol for Eclipta alba (L.) Hassk.: an endangered, medicinally important plant
Author(s): U. Salma, S. Kundu, Md. N. Ali and N. Mandal
Abstract: An in vitro protocol has been established for large-scale clonal propagation and conservation of Eclipta alba. Single nodal segment (NS) was used as an explant source for in vitro culture establishment, in which Murashige and Skoog (MS) medium fortified with 1.0 mgl-1 N6-benzyladenine (BA) and 0.25 mgl-1á-napthalene acetic acid (NAA) supported rapid axillary bud induction. A very elevated rate of shoot multiplication (22 shoots per axillary bud) was attained in the same growth medium within 21 days. A highest number of 15 well-developed roots per shoot were noted in MS medium containing 1.5 mg l”1 indole-3-butyric acid (IBA). The in vitro raised plantlets were well acclimatized in coco pith and soil (3:1) with the survival frequency of 91 per cent. From a NS, 88 acclimatized plants were achieved within 60 days. The sustainable potentiality of multiple shoot culture for over 10 months assured the technology towards conservation of genetic resources.
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