Author(s): V. KUMAR AND S. CHANDEL

Abstract: Phylloplane microflora diversity of rose was assessed during the winter and summer seasons in year 2015. Eight fungal flora i.e. Fusarium sp., Botrytis sp., Cladosporium sp., Penicillium sp., Aspergillus sp., Alternaria sp., Trichothecium sp., Trichoderma sp. and two bacterial isolates (Bacillus sp. and Pseudomonas sp.) were isolated from the phylloplane of the rose leaves in both seasons. The highest frequency of microflora occurrence in all months of summer and winter seasons were Fusarium, Aspergillus and Penicillium and bacterial isolates (Bacillus sp. and Pseudomonas sp.), while the Cladosporium sp. had observed only in the month of June. Further this isolated microflora was evaluated against the powdery mildew fungus under in vitro conditions by using slide spore germination test. The in vitro studies revealed that all the culture filtrate were effective against the rose powdery mildew. The highest conidial germination inhibition of 21.88 per cent was recorded from the culture filtrate of Trichoderma sp. followed by 16.20 per cent of Alternaria sp., Botrytis sp. (11.58%), Bacillus sp. (11.45%), Trichothecium sp. (10.89%) and Fusarium sp. (8.68%). The non effective culture filtrates with minimum conidial inhibition (2.66%) were Aspergillus sp. followed by Pseudomonas sp., (5.08%), Penicillium sp. (5.44%) and Cladosporium sp. (7.98%).

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