An experiment was conducted in Mathura Tea Garden at Alipurduar which is under Terai agro-climatic zone of West Bengal to evaluate efficacy of Glyphosate 41% SL to control weeds in Tea crop in the year 2015-2016. The design used in the experiment was Randomised Block Design with 8 treatments replicated thrice. The results observed revealed that treatments had no significant effect on the total fungi, bacteria and actinomycetes population in the soil. Despite the given treatments, the data showed rise in their population, even higher than the initial recorded population. It was also observed that Glyphosate 41% SL @3.00 l ha- 1 recorded the minimum population and dry weight of all categories of weeds. It can also be noted that the maximum weed control efficiency was found in Glyphosate 41% SL @ 3.00 l ha-1 at all the observations taken at 15 days interval followed by lower dose of the same and paraquat dichloride 24% SL @4.25 l ha-1. Maximum green leaf yield (12.37 q ha-1) was also recorded with Glyphosate 41% SL @ 3.00l/ha .Besides all these, application of Glyphosate 41% SL at lower and higher doses (2.00 and 6.00 l ha-1 respectively) didn’t cause any phytotoxic effect on the crop. Hence, it can be concluded that Glyphosate 41% SL@ 3.00 l ha-1 could be used for safe and effective control of weed in tea at Terai region of West Bengal.