Vol. 16 No. 1
Encapsulation-dehydration based cryopreservation of Rauwolfia shoot tips and their regeneration potential in vitro
Author(s): P. KISKU, S. SAHU, U. SALMA, S. SINHA RAY, P. SAHA, S. KUNDU AND MD. N. ALI
Abstract: The glory of R.serpentine has faced its alarming stage due to over exploitation of natural resource by the pharmaceutical industries. Hence a conservation approach is necessary to keep them alive in nature. Cryopreservation of synthetic seed based approach of in vitro conservation is most convenient and safe method for long term germplasm conservation. For this reason, an in vitro germplasm conservation method through encapsulation dehydration followed by cryopreservation has been developed for this plant using the shoot tip as explants. The optimal dose of sucrose during preculture of encapsulated seeds and the dehydration duration using laminar airflow has been tested during experiment. During dehydration, moisture content was found least after 7 hours of dehydration (6.78±0.93). After cryopreservation, maximum responses of seeds were recorded for pre-treatment at 0.3 M sucrose followed by 9 hours dehydration and 0.5 M sucrose followed by 7 hours dehydration respectively. Addition of RAPD based approach suggested there was no variation between the mother plant and cryopreserved plant after regeneration.
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