Vol. 5 No. 2
Regeneration of plants from hypocotyl derived callus tissue of jute (Corchorus olitorius L. var. JRO-632).
Abstract: The present paper deals with the induction and regeneration of callus tissue from in vitro system of jute (Corchrous olitorius L. Var JRO-632). Hypocotyl of in vitro germinated seedlings was taken as explants. Hypocotyl explants were cultured in both Murashige and Skoog’s (1962)(MS) and Schenk and Hildebrand’s (1972)(SH) basal media supplemented with different growth regulators in definite concentrations. Callus growth was found better in MS basal medium than SH medium. For morphogenetic study the hypocotyl callus tissues were grown in medium containing different combinations and concentrations of auxins (IAA, NAA, IBA and 2,4-D used separately @ 0.20 mg/L) and cytokinins (BAP and Kinetin used separately 0.15mg/K to 3.2 mg/L, coconut milk 10-35% volume/volume). A simplified method for morphogenesis of calli was standardized. Cytological studies of the regenerated roots from calli as well as from regenerated plants revealed diploid chromosome number while the calli were mixoploid in nature. It was observed that the balance in the concentration of auxin-cytokinin (in initial culture) as well as ploidy level in the cells is most important-factors in controlling differentiation.
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