Vol. 8 No. 2
Effect of gamma rays and ethyl methane sulphonate on germination, pollen viability and survival of okra [Abelmoschus esculentus (L.) Moench]
Author(s): P. A. JADHAV, H. V. KALPANDE, M. N. KATHALE AND G. P. DAHALE
Abstract: A study was conducted in Parbhani, Maharashtra, India, during kharif 2010 and summer 2011 to investigate the effect of mutagenic treatment on biological characters of okra cv. Parbhani Kranti in M1 and M2 generations. Two mutagens, i.e. gamma rays (15, 30, 45 and 60 kR) and ethyl methanesulfonate (EMS, 0.2, 0.4, 0.6, 0.8 and 1.0%), were used. Gamma ray and EMS treatments decreased the germination of okra in the M1 generation compared to the controls. In the M2 generation, germination increased in all the treatments. The highest mortality was recorded at 60 kR gamma rays in the two progenies of okra. Pollen sterility increased in all the treatments compared to their controls in both the generations. In the M1, the highest pollen sterility was recorded at 45 kR gamma ray. Higher doses of gamma rays and EMS had deleterious effects on seed germination, pollen fertility and plant survival. The EMS treatment caused more physical damage than the gamma ray.
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