Vol. 9 No. 1
Development of SSR markers in mung bean, Vigna radiata (L.) Wilczek using in silico methods
Author(s): N. SINGH, H. SINGH, P. NAGARAJAN
Abstract: Nucleotide sequences available in public database provide a cost effective and valuable source for the development of molecular markers. In this study, the nucleotide sequence database available in National Centre for Biotechnology Information (NCBI) is utilized to identify and develop SSR markers in mungbean (Vigna radiata). A total of 803 genomic sequences, 829 EST sequences and 82 GSS sequences were downloaded from NCBI. Eight hundred and forty two SSRs from genomic sequences, 240 SSRs from EST sequences and 60 SSRs from GSS sequences were obtained using SSRIT tool. Primers pairs were successfully designed for 109 SSR motifs from genomic sequence, 110 SSR motifs from EST sequence and 25 SSR motifs from GSS sequences using Primer3 (http://frodo.wi.mit.edu) software. Fifteen SSR primers were finally characterized and validated in 24 mungbean and six urd bean accessions.
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