Vol. 12 No. 1
Variation in peroxidase banding pattern as response to boron deficiency in wheat (Triticum aestivum Thell am)
Author(s): B. DAS, T. BANDOPADHYAY, S. MUKHERJEE, B. PRIYA AND K. K. SARKAR
Abstract:
Widely used isozyme had been employed for identification of variations and estimation of genetic diversity within genotypes in a population and also successfully used in various crop improvement programme. Among the markers peroxidase had been reliably helpful in genetic studies and planning appropriate breeding strategies for improvement of many crops including wheat. The present study was conducted for characterization of some boron–deficiency tolerant and susceptible genotypes following PAGE technique. The tolerant genotypes Scholburgk, DBW 14, Halna and boron –deficiency susceptible genotypes PBW 343, WH 736, HD 2733 (Res.) were considered in the present investigation. Peroxidase profile revealed marked differences within tolerant and susceptible genotypes with respect to number of bands, Rm values and intensity of gels. Out of six bands, bands with Rm value 0.83 was absent in two susceptible genotypes WH 736 and HD 2733 and band with Rm value 0.283 was found absent in another susceptible genotypes PBW 343. Other remaining bands were present in all the genotypes irrespective
of their tolerance or susceptibility and all the six bands were found present in the tolerant genotypes.

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