Vol. 13 No. 1
Micropopagation of Anthurium andreanum cv. Jewel from leaf explants
Abstract: A protocol for the mass propagation of Anthurium andreanum cv. Jewel has been developed through callus induction and organogenesis using leaf lamina as explant. MS basal medium containing 3 mg L-1 2,4-D + 0.5 mg L-1 BAP + 0.4 mg L-1 TDZ took minimum number of days (37.68) to initiate callus. However, maximum callus production was observed in the media containing 2 mg L-1 NAA (77.33%). Young leaves exhibited better responses and the calli were compact and dark green. The shoots were initiated later from the calluses by inoculating on MS media containing 3.0 mg L-1 BAP + 0.5 mg L-1 NAA which took a minimum of 27.83 days while MS + 2 mg L-1 BAP + 0.5 mg L-1 NAA gave highest regeneration percentage (98.89%) as well as shoot length of 2.23 cm. Maximum number of shoots per callus (5.83) and highest number of shoots following second subculturing (22.60) were observed when MS basal medium was fortified with 2 mg L-1 BAP singly. For root induction, 1/2MS media supplemented with 2.0 mg L-1 NAA took the least days (33.55). Maximum root induction per plant (5.83) and length (4.28 cm) of root were recorded in 1/2MS + 1.0 mg L-1 NAA. The well rooted Anthurium plantlets were successfully hardened in coconut husk with highest survival percentage of 96.17 per cent at vermiculite + coconut husk (1:1, w/w).
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