Vol. 15 No. 1
Rapid and consistent QuEChERS based RP-HPLC methodology for the detection of various insecticides in cabbage and cauliflower
Author(s): R. AKOIJAM AND R. S. TELEM
Abstract: A rapid and reliable reverse phase high performance chromatograph (RP-HPLC) method was developed for the instantaneous detection of insecticides residues of imidacloprid, carbofuran, chlorantraniliprole, fipronil, malathion, chloropyriphos and cypermethrin in cabbage and cauliflower. Modified QuEChERS method with the inclusion of primary secondary amine (PSA) sorbent and grafitized carbon black were used for extraction and clean-up process. The separation of insecticides was performed by C18 column with UV-VIS detector. The dual pump functions as isocratic flow of mobile phase of acetonitrile and water (90:10, v/v) with the flow rate of 0.5 mL min-1. The peaks of imidacloprid, carbofuran, chlorantraniliprole, fipronil, malathion, chloropyriphos and Cypermethrin A, Cypermethrin B and Cypermethrin C in chromatograms observed at retention times of 2.66 min, 3.25 min, 3.54 min, 4.03min, 4.17 min, 8.63 and 9.94, 10.46 and 10.67 mins respectively. The average recoveries were found above 80 per cent for all the insecticides in both the vegetable samples when the samples were spiked at different levels of 0.05, 0.10, 0.25, 0.50 and 1.00 mg kg-1 levels.
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