Vol. 15 No. 3
Detection and molecular characterization of Begomovirus infecting some common weeds in West Bengal
Author(s): S. CHAKRABORTY, T. ABING, S. DAS AND J. TARAFDAR
Weeds are often overlooked for their role as a potential carrier of several viruses. In our present study symptomatic weeds such as Croton sparsiflorus, Acalypha indica, Sida rhombifolia, and Ageratum conyzoides, were collected and detected through PCR by using universal and begomovirus specific primers. Begomovirus infecting Croton sparsiflorus and Acalypha indica showed a maximum similarity of 97.94% and 97.04% respectively to Jatropha leaf yellow mosaic virus (NC_038956), and begomovirus infecting Sida rhombifolia and Ageratum conyzoides showed maximum similarity of 96.84% with Cotton ileaf icurli Bangalore virus (KT390464.1), and Ageratum ienation ivirus isolate Gorakhpur (GQ268327) (95%) respectively. Further phylogenetic analysis and amino acid structure prediction were performed to determine the relationship with the existing begomovirus in the world, that showed variation in sequence diversity and expansion of population among the isolates infecting the weeds. The present outcomes revealed that weed species may play a significant role in harboring various begomoviruses and may play a crucial role in recombination with different begomoviruses.
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