Vol. 16 No. 3
Assessment of genetic diversity and molecular detection of advanced inbred lines for QPM status in maize (Zea mays L.)
Author(s): D. LENKA, D. SWAIN, DEVIDUTTA LENKA AND S. K. TRIPATHY
Abstract: A set of 50 inbred lines derived from diverse QPM populations were subjected to multivariate analysis for genetic divergence based on 15 quantitative traits. The test inbreds were grouped into 10 and 11 clusters following Tocher’s and Canonical method. Clustering pattern was almost similar in both these methods. Days to 75% dry husk contributed maximum to genetic divergence (29.9%) followed by days to 50% tasseling (15.2%). VL1016556 is considered to be most divergent inbred followed by VL1016352, QPM-8-3-2 and QPM-1-15-1 based on D2 values. The top three high yielding inbreds (QPM-2-15, QPM 11-7-1 and VL1016453) were clubbed into the Cluster VIII with maximum mean seed yield. Cluster VIII maintained maximum genetic distance from Cluster X. Besides, Cluster IV and X, IV and IX, IV and VII were sorted out as diverse cluster pairs and these could be useful for obtaining high heterotic hybrids. The QPM allele specific marker revealed more discriminating power (Rp=1.8) among the genotypes compared to umc 1066 (Rp=1.2). Among the selected 20 inbreds, QPM 11-7-1, QPM 7-3-2, QPM 8-3-2, QPM 10-13- 1, VL1016414, VL109476, VL109475, VL1016399, VL1017054, VL109412 and VL1016590 revealed the 169bp QPM allele and hence, these may be considered as potential QPM inbred lines. These inbred lines may serve as valuable material for QPM breeding.
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