Vol. 17 No. 2
Molecular characterization of sunflower advanced inbred lines derived from powdery mildew resistance breeding programme
Author(s): L. GANGAVATI AND V. KULKARNI
Abstract: Sunflower, an important oilseed crop ranks second as a source of edible oil at the global level. In order to derive hybrids producing high oil and achene yield, potential and distant inbred lines need to be produced to take advantage of commercial heterosis. Evaluation of genetic diversity of such parental lines and assessment of zygosity in hybrids using various molecular markers is very important in sunflower hybrid breeding programs. In the present study, 121 sunflower genotypes including 117 advanced inbred lines derived for powdery mildew resistance and 4 checks were genotyped using 30 microsatellite markers. The number of alleles determined varied from 2 to 3 alleles/locus. Average number of alleles per locus were 2.1. The most powerful markers on the basis of PIC value were ORS938 (0.5) and ORS 1088 (0.5) whereas the least powerful marker was ORS309. The molecular diversity analysis grouped 121 sunflower inbred lines into sixteen main clusters indicating moderate amount of diversity among them at 0.72 Jaccard’s coefficient of similarity. Careful selection of diverse inbred lines would help in the hybrid development programs by exploiting commercial heterosis.
Download Full Article: Click Here