A study was conducted during two consecutive kharif seasons of 2019 and 2020 to standardize the shade requirement and method of harvesting for obtaining high essential oil and eugenol contents in tulsi. Experiments were laid out in split plot design with two main plots and four sub plot treatments. Growing of tulsi under 50 per cent shade and harvesting at 20 - 30 cm height from ground level at 75 and 135 days after transplanting (DAT) can be recommended for higher essential oil production. For open growing condition, harvesting at 20 - 30 cm height at 90 and 150 DAT can be recommended for higher eugenol content.