Vol. 18 No. 2
A spectrum of chemical tests in rice varieties identification and classification
Author(s): GBN JYOTHI AND S. DAS
Abstract:
Investigation used a variety of chemical assays to identify and characterize rice varieties. Seed samples of Sixteen rice varieties were used in experimentation which are under cultivation in the state of Odisha. These were released by Odisha University of Agriculture and Technology, Bhubaneswar and National Rice Research Institute, Cuttack. Phenol test clustered sixteen varieties into six distinct groups viz., very pale brown (1 variety), pale brown (5 varieties), brown (5 varieties), deep brown (1 variety), very deep brown in color (3 varieties) and no color change (1 variety). Modified phenol test using CuSo4 solution helped in further sub-division of standard phenol group into four sub-groups viz., pale brown (5 varieties), brown (7 varieties), deep brown (2 varieties), and black color (1 variety). Based on NaOH test varieties grouped into three classes such as no color(1 variety), pale yellow (8 varieties) and yellow (7 varieties). In case of KOH test, varieties grouped into six classes such as no color (1 variety), pale yellow(2 varieties), yellow (3 varieties), pale brown (5 varieties),brown (3 varieties)and deep brown or reddish brown (2 varieties).The genotypes were categorized based on their distinct responses to GA3 in terms of coleoptile length growth as very low response (< 10 %), low response (10-30 %) with eight genotypes and moderate response (>30 %) with eight genotypes. The increase in coleoptile length over control ranged from 14.03 per cent (Jogesh) to 48.87 per cent (Manaswini).
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