Field trial was laid for two successive years in 2019-20 and 2020-21 in TypicUstochrept soil of neutral reaction (pH 7.1) with medium organic carbon (OC) status (5.51 g kg-1) and having available N, P, K: 296, 33 and 152 kg ha-1 at Nilganj, North 24 Parganas, West Bengal for understanding the consequence of dose of primary nutrients and application methods of fertilizer (especially N) on the seed yield, seed quality and profitability in tossa jute (Corchorus olitorius L.) (cv. JRO 204) in the intensively jute fibre growing southern Bengal districts. The peak jute seed yield of 1103 kg ha-1 was obtained withT7[i.e., N 80 (in 3 splits of ½ at 21-28 DAS, ¼ at 42 DAS and ¼ at 56 DAS), P2O5 40, K2O 80 kg ha-1];and 3 split applications of N gave 7.6% more seed yield. Regarding jute seed quality, the same T7 treatment produced seeds of the highest test wight (2.294 g) with the maximum vigour index (2205). Application of N, P, K gave positive balance of available nutrients of 40-57, 5 and 27 kg ha-1, respectively N in 3 splits resulted higher agronomic efficiency of applied nutrients to the tune of 8.21 to 16.81% more as compared to the 2 split applications. The highest net return from the jute seed production was ` 87,294 ha-1 and the highest net return per rupee investment (NRPRI) was 1.73 in N80 (3 splits), P40 and K80 treatments.