Vol. 18 No. 3
EMS induced mutagenesis in green gram for small seeded bruchid resistant genotype
Author(s): A. SEN, A. P. SINGH, S. SARKAR AND S. BHATTACHARYYA
Abstract:
Present investigation was conducted in green gram variety PM5. It is a high yielding, bold seeded as well as highly resistant to bruchid, a devastating coleopteran storage pest. The seeds of that variety were exposed to near LD50 concentration of EMS to induce mutation. In comparison to the untreated PM5 plants, different types of phenotypic variations were observed in M1 generation. In M2 generation, many putative mutants were identified. Small seededness in few M2 plants was the most significant finding in the present study. It is noteworthy to mention that, except one plant, rest of those M2 plants showed decrease in bruchid resistance as revealed by the M3 seeds feeding assay.
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