Volume 10 Issue 2

Fate and behaviour of azoxystrobin in chilli by using liquid chromatography with mass spectroscopy

Author(s):S. Ghosh, S. Mukharjee, A. Bhattacharyya, A. Bhattacharyya and S. Roy

Abstract: A liquid chromatography mass spectrometry (LC/MS-MS) method for analysis of residual azoxystrobin in chilli was developed in present work. Azoxystrobin is generally a systemic fungicide applied in chilli to control anthracnose and powdery mildew diseases. The method was based on extraction with 1:1 ethyl acetate: 10% aq. NaCl solution, clean-up by PSA, GCB and florisil, evaporated in N2 evaporator, followed by volume make up with Acetonitrile and analysed by LC/MS-MS in multiple reaction monitoring (MRM) mode. Recoveries from fortified samples were found in the range from 90% to 92%. The limit of quantification (LOQ) of the method was 0.01 ppm. The half life value of azoxystrobin in chilli was determined as 0.73 to 0.80 days......

Fig. : Calibration curve of azoxystrobin

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