Volume 5 Issue 1

In vitro mass multiplication with genetic clonality in
elephant garlic (Allium ampeloprasum L.)

Author(s): S GANTAIT, N. MANDAL, S. BHATTACHARYYA AND P. K. DAS
Abstract: A novel protocol was developed for Allium ampeloprasum L. to enhance in vitro cloning through multiple shoot induction. First bud induction was recorded from shoot tip within 6 days in MS with 0.25 mgl^-1 NAA and 2 mgl^-1 Kn. A maximum of 3 buds from a single explant appeared within the 15 days after the first bud induction. MS with 2.5 mgl^-1 Kn plus 60 mgl^-1 of adenine sulphate proved best for multiple shoot proliferation resulting in 6 shoots per inoculated shoot bud within next 30 days. Maximum 5 roots per shoot were recorded when cultured on MS with 0.5 mgl^-1 IAA for 20 days. The combination of soil, sand and vermicompost with intermittent water spraying proved to be the best for hardening of micropropagated plantlets ensuring 90% success in next 25 days. Selected ISSR primers were used to ensure genetic clonality for the in vitro generated propagules.

Fig: A- Bud induction, B- Shoot multiplication, C- Root induction and elongation, D- Primary acclimatization, E- Secondary acclimatization, F- Gel electrophoresis of amplified ISSR products of in vitro regenerated propagules (C1 – C5) and their mother (P) using IS-8 primer. Lane M- 50bp ladder.

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