Vol. 15 No. 2
Sterilization, germination and shoot proliferation from Chrysanthemum coronarium L. seeds
Abstract: Chrysanthemum coronarium, popularly called as garland chrysanthemum, is an annual highly cross-pollinated species and propagated by seed. The different flower forms viz., single, semi-double and double types are available in the species. However, maintaining such types is difficult due to high self incompatibility and heterozygosity due to outcrossing. Therefore development of homozygous lines is not possible by conventional methods due to segregation of progenies. Micropropagation offers production of large number of disease free, true to type planting material within short period of time irrespective of growing season for cultivation per se or for complementing crop improvement programme. Aseptic condition of culture medium is one of the main aspects for micro propagation. It was achieved by sterilization procedures using chemical treatments. Out of different surface sterilants pre tried, it was evident that the maximum seed germination (73.31 %) was obtained when seeds were surface sterilized with carbendazim (0.2%), ridomil (0.2%) and hydroxy quinoline citrate (8-HQC) (200 ppm) for 2 hours followed by ethanol (70%) for 30 sec and HgCl2 (0.1%) for 3 minutes. Further, the seeds cultured on half strength Murashige and Skoog (MS) medium and gibberelic acid (GA3 -) (2.0 mg L-1) resulted in highest seed germination (51.34 %). Highest number of shoots micro-shoot-1 was obtained when the microshoots were sub cultured on half strength MS medium and GA3 (0.5 mg L-1).
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