Author(s): P. DATTA, B. DASGUPTA AND D. K. SENGUPTA

Abstract: Seventeen isolates of Trichoderma spp. isolated in Trichoderma Specific Medium (TSM) form soils of different betel vine plantations of West Bengal and two isolates form soils of Andhra Pradesh and Madhya Pradesh. Cultural, morphometric and antagonistic potential against Phytophthora parasitica (4 isolates) and Pythium spp. (6 isolates) of each Trichoderma spp. isolates were studied. The morphometric studies revealed that the highest phialides, phialospores, chlamydospore were recorded in isolate T13 and length of conidiophore was reordered highest in T9, T4, T13and T12 isolates respectively. The chlamydospores recorded were in twins, chains or in intercalary in positions. The antagonistic potential of all the isolates of Trichodermaspp. were tested by Dual Plate Technique against all the isolates of Phytophthora parasitica and Pythium spp. and one isolate of Phytophthora parasitica were tested by inverted plate technique to find out the volatile properties against all the isolates ofTrichoderma spp. From the cultural and morphometric characters, it was revealed that among the 17 isolates of Trichoderma spp., T6, T7, T9, T10, T11, T12, T13 and TJN areTrichoderma viride, T4 is T. virens and rest isolates are T. harzianum. The results of antagonistic property revealed that T4, T7, T12, T14, TB and TJN showed highest promise under in vitro conditions (Dual Plate Technique) by fully overgrowing the pathogens as reordered by Bell\'s scale within 7-8 days. Isolates T7, T13, T15 and T4 ofTrichoderma spp. showed some promise against isolate of Phytophthora parasitica by inverted plate technique within 4-5 days by releasing some volatiles. From the results, it could be concluded that isolates T4 and T7 were highly effective against the above pathogen by both the techniques tested..

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