Vol. 9 No. 1
In vitro regeneration in snake gourd (Tricosanthes anguina) from shoot apical meristem and young leaves
Author(s): M. E. HAQUE, D. REZWANA, M. I. ISLAM, AND B. SIKDAR
Abstract: Snake (Tricosanthes anguina) gourd is a popular vegetable and medicinal plant in Bangladesh. The present investigation was under taken for in vitro regeneration of snake gourd from shoot apical meristem and juvenile leaves in Murashige and Skoog (1962) medium supplemented with various concentration and combination of BAP, 2,4-D, NAA, IBA and GA3. Among different concentrations, 1 mg l-1 BAP was found for effective callus induction from shoot apical meristem and 2.5 mg l-1 2, 4-D+0.5 mg l-1 BAP from leaves. The highest result for shoot regeneration was observed from meristem derived calli using 1.5 mg l-1 BAP+0.2 mg l-1GA3 and 2.5 BAP+0.2 mg l-1GA3 from leaves derived calli. In vitro regenerated shoots were rooted well in 1/2 strength MS with 0.5 mg l-1 NAA and well rooted plantlets were successfully established in pot soil with 80% survival rate.
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