Vol. 14 No. 1
In vitro and in vivo assessment of Thidiazuron mediated micro-clones of Dendrocalamus asper, an ornamental bamboo species
Author(s): S. S. RAY, MD. N. ALI, M. BANERJEE AND L. YEASMIN
Abstract: Dendrocalamus asper is an economically important ornamental as well as edible bamboo species. Long flowering cycle limits its propagation which has been surmounted a little by macropropagation. Tissue culture has already been proved as efficient method for large scale propagation. Despite the higher potentiality of thidiazuron as promising phytohoromone, no report is available in micropropagation of D. asper. A complete protocol of thidiazuron mediated micropropagation of D. asper is reported for the first time in the present report. Murashige and Skoog (MS) media with very low concentrations of thidiazurone (0.25 and 0.5 mg/l) were found equally effective with higher concentrations (3 and 4.5 mg/l) of 6-Benzyladenine (BA) for bud breaking and shoot length. Significant multiplication was observed when the single shoot from thidiazuron containing solid MS, was transferred to liquid MS medium supplemented with 3 mg l-1 BA, which is rarely possible in case of BA. Both root length and root numbers/plant was found maximum at ½MS with 1 mg l-1 IBA (Indole-3-butyric acid) during root induction. Simple cost effective hardening procedure using cocopeat (100%) followed by soil (100%) promoted high survivability during acclimatization. Healthy plantlets with higher survival rate were ascertained from a field trial of 18 months.