Vol. 17 No. 3
Molecular characterization of whitefly (Bemesia tabaci Genn.) and development of management module against chilli leaf curl complex
Abstract: Chilli leaf curl is the major constraint in chilli cultivation which is associated with the infestation of thrips, yellow mites and whitefly mediated leaf curl virus. Now a days chilli leaf curl virus is spotted as threat to chilli growers as most of the potent chemical in solo application found unsuitable for the management of whitefly biotype Q. Attempts have been made to address the issues of chilli leaf curl complex, molecular characterization of whitefly biotype and development of its effective management module. Experimental findings of molecular identification of different adult Bemisia tabaci samples collected across five agro-ecological zones of West Bengal, India revealed that out of 11 samples collected from different location samples from 8 locations resembles biotype Q based of amplicon pattern using Bem 23 microsatellite marker and samples from other 3 locations showed a little genetical diversity. Based on our present findings seed treatment with thiamethoxam 70 WS 3 g kg-1 seed incorporated with seedling treatment with acetamiprid 20 SP @ 1g l-1, seedling raising under insect proof net of 80 mesh size and border netting with insect proof net showed significant impact in reducing the occurrence and dispersal of thrips (Scirtothrips dorsalis Hood), mite (Polyphagotersonemus latus) and whiteflies. Lowest population (per three leaves) of thrips (1.92), whiteflies (1.14) and mites (2.30) was recorded by IPM module (integration of seed treatment, seedling treatment, seedling raising under insect proof net, border netting technology, installation of yellow sticky trap and need based spot application of spiromesifen and diafenthiuron) with 92.97%, 82.68%, 72.97% reduction, respectively. Reduction of chilli leaf curl virus (CLCV) incidence was also highest (98.56%) through IPM with maximum yield of green chilli (1.66 t ha-1). Panchagavya, dasaparni and bhramvastra appeared as potent bio-pesticides in reducing the CLCV causative agents. The residue analysis result showed that the pesticides used were below the instrumental LOQ range.
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